Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Long non-coding RNA LINC00470 regulates the growth and epithelial-to-mesenchymal transition of human oral cancer cells

Fengjia Liu , Min Liu, Wannan Li, Yuejian Ou

Stomatology Therapeutic Centre, Huzhou Central Hospital, Affiliated Centre Hospital Huzhou University, Huzhou 313000, PR China;

For correspondence:- Fengjia Liu Email: 18559861@qq.com

Accepted: 28 October 2022 Published: 30 November 2022

Citation: Liu F, Liu M, Li W, Ou Y. Long non-coding RNA LINC00470 regulates the growth and epithelial-to-mesenchymal transition of human oral cancer cells. Trop J Pharm Res 2022; 21(11):2331-2336 doi: 10.4314/tjpr.v21i11.9

© 2022 The authors.
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Abstract

Purpose: To investigate the role of long non-coding RNA LINC00470 in human oral cancer tissues and cell lines (OC3, SCC9 and CAL27).

Methods: expression of mRNA was determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Cell viability and proliferation were measured using MTT and EdU assays, respectively, while cell migration and invasion were determined with transwell assay. Protein expression was assayed with western blotting.

Results: There was significant up-regulation of LINC00470 in the cancer tissues and cell lines (OC3, SCC9 and CAL27) (p < 0.05). Silencing of LINC00470 led to significant inhibition of the proliferation and colony-formation potential of the oral cancer cells (p < 0.05). Furthermore, silencing of LINC00470 in oral cancer cell lines caused significant induction of apoptosis, suppression of migration and invasion, and restoration of epithelial-to-mesenchymal transition (EMT).

Conclusion: These results strongly demonstrate the oncogenic role of LINC00470 in oral cancer, thereby suggesting its possible prognostic and therapeutic potential in this lethal malignancy.

Keywords: Oral cancer, Long non-coding RNA, LINC00470, Apoptosis, Metastasis, EMT